Blood rune

Blood runes are the second highest-level runes, and the most valuable runes of all. They are used in the highest level spells, the lowest of which being Ruby Crossbow Bolt Enchant, and the highest being Ice Barrage. They cannot be made through the Runecrafting skill, but they can be obtained through other ways, including:
 * Purchase from various Rune shops, including:
 * Magic Guild shop
 * Ali Morrisaine's Runeshop (after The Feud quest and part of Rogue Trader mini-quest)
 * Looting from the Barrows' Chest
 * Thieving from the Chaos Druid's Tower chest (requires level 59 Thieving)
 * As drops from various monsters

Blood runes are used in all of the following spells in the Standard Spellbook: The Ancient spellbook makes extensive use of Blood runes, and they are used in exactly half of all Ancient spells: The Lunar spellbook uses Blood runes only in its healing spells - Heal Other (Level 92) and Heal Group (Level 95).
 * Enchant Ruby Crossbow Bolts (Level 49)
 * Saradomin Strike (Level 60)
 * Claws of Guthix (Level 60)
 * Flames of Zamorak (Level 60)
 * Wind Wave (Level 62)
 * Water Wave (Level 65)
 * Earth Wave (Level 70)
 * Fire Wave (Level 75)
 * Charge (Level 80)
 * Blood Rush (Level 56)
 * Kharyrll Teleport (Level 66) (Canifis)
 * Blood Burst (Level 68)
 * Smoke Blitz (Level 74)
 * Shadow Blitz (Level 76)
 * Blood Blitz (Level 80)
 * Ice Blitz (Level 82)
 * Smoke Barrage (Level 86)
 * Shadow Barrage (Level 88)
 * Annakarl Teleport (Level 90) (Demonic Ruins)
 * Blood Barrage (Level 92)
 * Ice Barrage (Level 94)